Technology Development
Our research has a long-standing history of developing or refining new computational and or experimental solutions to identify new functionalities in the human genome. Computationally, we have developed solutions for RNA-sequencing in bulk and single cell populations, fRIP-sequencing and one of the first machine learning approaches to identify functional polymorphisms in the human population. We have refined numerous computational approaches to develop lncRNA catalogs, characterization of global lncRNA properties and live cell image tracking of chromosomal movements.
Experimentally we have developed several new approaches to gain unique insights into the human genome. This includes PIP-seq, fRIP-seq, and MPRNA towards our ultimate goal of understanding the molecular syntax of lncRNA biology. We have also developed two distinct applications of CRISPR-CAS9 systems. Specifically, CRISPR-Display that serves as a drone to carry RNA cargo to specified regions of the genome. As well as live cell imaging approaches such as CLING-FISH and SNP-CLING (Crispr Live Cell Imaging) to monitor how chromosomes fold and interact in living cells.